Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid column heating method for subcritical water chromatography.

Michael O Fogwill1, Kevin B Thurbide

  • 1Department of Chemistry, University of Calgary, 2500 University Drive N.W., Calgary, Alberta T2N 1N4, Canada.

Journal of Chromatography. A
|November 28, 2006
PubMed
Summary

A new resistive heating method significantly speeds up subcritical water chromatography (SWC) column heating, reducing elution times by up to 50% for faster separations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Dual Column pH Switchable Water Stationary Phase System for Separation Control in Supercritical Fluid Chromatography.

Journal of separation science·2024
Same author

A novel switchable water stationary phase for supercritical fluid chromatography.

Analytica chimica acta·2023
Same author

Sulfolane as a novel stationary phase for analytical separations by gas chromatography.

Analytica chimica acta·2021
Same author

Turbulent Supercritical Fluid Chromatography in Open-Tubular Columns for High-Throughput Separations.

Analytical chemistry·2020
Same author

Active control of selectivity in organic acid analysis by gas chromatography.

Analytica chimica acta·2020
Same author

Dynamic Control of Gas Chromatographic Selectivity during the Analysis of Organic Bases.

Analytical chemistry·2019

Area of Science:

  • Analytical Chemistry
  • Chromatography Science

Background:

  • Subcritical water chromatography (SWC) uses temperature to control water's polarity for gradient elution.
  • Conventional heating methods in SWC, like gas chromatography (GC) ovens, limit heating rates and thus gradient effectiveness.

Purpose of the Study:

  • To develop and evaluate a novel resistive heating method for SWC.
  • To achieve significantly higher column heating rates compared to traditional GC convection ovens.

Main Methods:

  • A separation column was wrapped with ceramic insulation and a resistively heated wire.
  • The prototype resistive heating system was tested in a subcritical water chromatography setup.
  • Heating rates and solute retention times were compared to a conventional GC convection oven.

Related Experiment Videos

Main Results:

  • The resistive heating method achieved five-fold higher column heating rates than conventional GC ovens.
  • Maximum heating rates increased from approximately 10°C/min to over 50°C/min.
  • Elution times for alcohol test mixtures decreased by 35% to 50% for the latest eluting component.
  • Solute retention times showed less than 1% relative standard deviation (RSD) over multiple trials.

Conclusions:

  • The novel resistive heating method offers a substantial improvement in heating rates for SWC.
  • This technique enables faster gradient elution and reduced analysis times in SWC.
  • The developed method is a viable alternative for enhancing heating efficiency in SWC applications.