Related Experiment Video
Updated: Jul 18, 2026

Stable Isotope In-Vivo Labeling for Mass-Spectrometry Identification of Paternal Metabolites Transferred from Sperm to Oocyte During Fertilization
Published on: June 17, 2025
Collection and freezing of epididymal stallion sperm
1Department of Animal Sciences, Equine Reproduction Laboratory, Animal Reproduction and Biotechnology Laboratory, Colorado State University, 3194 Rampart Road, Fort Collins, CO 80523 USA. jason.bruemmer@colostate.edu
Abstract:
The ability to harvest and preserve epididymal sperm from a stallion after simple elective castration, a catastrophic injury, or severe acute illness and subsequent death has been realized, allowing for the preservation of genetics that would have been lost otherwise.Currently, the care taken to collect the testes and epididymides properly, coupled with proper packaging and shipping, could make the greatest contribution to salvaging viable sperm. As advances in assisted reproductive techniques continue, more offspring may be obtained from stored epididymal sperm from valuable stallions.

