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Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer
Published on: April 27, 2020
From Never Born Proteins to Minimal Living Cells: two projects in synthetic biology
Pier Luigi Luisi1, Cristiano Chiarabelli, Pasquale Stano
1Biology Department, University of RomaTre, Viale G. Marconi 446, 00146 Rome, Italy. luisi@mat.ethz.ch
Abstract:
The Never Born Proteins (NBPs) and the Minimal Cell projects are two currently developed research lines belonging to the field of synthetic biology. The first deals with the investigation of structural and functional properties of de novo proteins with random sequences, selected and isolated using phage display methods. The minimal cell is the simplest cellular construct which displays living properties, such as self-maintenance, self-reproduction and evolvability. The semi-synthetic approach to minimal cells involves the use of extant genes and proteins in order to build a supramolecular construct based on lipid vesicles. Results and outlooks on these two research lines are shortly discussed, mainly focusing on their relevance to the origin of life studies.
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