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Updated: Jul 18, 2026

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
Published on: February 26, 2018
Condensation of interphase chromosomes in nuclei of synchronized CHO cells
1Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen, Hungary. bgaspar@delfin.klte.hu
Abstract:
The escape of individual interphase chromosomes from nuclei of reversibly permeabilized Chinese hamster ovary (CHO) cells was utilized for the visualization of condensing interphase chromosomes in a cell cycle-dependent manner in synchronized cells. Major interphase chromosomal forms include: (a) mid-S-phase globular chromosomes at 3.0 C-value, (b) late mid-S-phase fibrous hemicircular forms (3.3 C), (c) late-S-phase supercoiled ribbons (3.7 C), and (d) end-S-phase elongated, bent prechromosomal structures (4.0 C).
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