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Related Experiment Video

Updated: Jul 18, 2026

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

Generation of conditional Cited2 null alleles.

Jost I Preis¹, Natalie Wise, Mark J Solloway

¹Developmental Biology Program, Victor Chang Cardiac Research Institute, Sydney, Australia.

Genesis (New York, N.Y. : 2000)

|November 30, 2006

Summary

Conditional null alleles for Cited2 (a transcriptional co-factor) were generated. These tools enable precise study of Cited2

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Last Updated: Jul 18, 2026

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Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

Area of Science:

Developmental Biology
Genetics
Molecular Biology

Background:

Cited2 is a crucial transcriptional co-factor for early embryonic development.
Germline Cited2 null embryos exhibit severe developmental defects, including organogenesis and body axis patterning.
Understanding Cited2's specific roles in different tissues requires targeted gene inactivation.

Purpose of the Study:

To generate and validate conditional null alleles for the Cited2 gene.
To enable Cre-recombinase-mediated somatic cell inactivation of Cited2.
To provide tools for dissecting Cited2 function in organ development and embryonic patterning.

Main Methods:

Generation of two conditional null alleles for Cited2.
Cre-recombinase-mediated somatic cell gene inactivation.
Crossing conditional mutants with CMV-Cre transgenic mice.
Analysis of lacZ expression under the Cited2 promoter.

Main Results:

Conditional Cited2 alleles allow for viable and fertile heterozygous/homozygous mice.
Somatic deletion of Cited2 using CMV-Cre recapitulates the embryonic-lethal phenotype of germline null mutants.
Conditional deletion effectively drives lacZ expression, indicating promoter activity.

Conclusions:

The generated conditional null alleles are effective genetic tools for studying Cited2.
These alleles facilitate the dissection of Cited2's function in specific organs and developmental processes.
The tools allow for temporal and spatial control of Cited2 gene inactivation during embryogenesis.