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Related Experiment Videos

D-factor: modulation of expression in fibroblasts.

T Miyagi1, M Akashi, K Yamato

  • 1Department of Medicine, Cedars-Sinai Medical Center, UCLA 90024.

Leukemia Research
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

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Fibroblasts constitutively express differentiation-inducing factor (D-factor) RNA. Expression increases with stimuli like tumor necrosis factor (TNF), indicating D-factor

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Immunology

Background:

  • Differentiation inducing factor (D-factor) is a recently identified protein with largely unknown gene expression regulation.
  • Fibroblasts are known to express various cytokine RNAs in response to stimuli.

Purpose of the Study:

  • To investigate the constitutive and inducible expression of D-factor RNA in fibroblasts.
  • To identify stimuli that regulate D-factor gene expression in mesenchymal cells.

Main Methods:

  • Analysis of D-factor RNA expression in fibroblasts from various tissues (lung, bone marrow, gingiva, foreskin).
  • Exposure of fibroblasts to stimuli including activated macrophage/lymphocyte products (TNF, IL-1, lymphotoxin), protein kinase C activators (TPA, teleocidin), G-protein activators (NaF), and protein synthesis inhibitors (cycloheximide).

Related Experiment Videos

  • Assessment of D-factor transcript stability following stimulation.

    • Main Results:

    • Fibroblasts from multiple tissues constitutively express D-factor RNA.
    • Stimuli such as TNF, IL-1, lymphotoxin, TPA, teleocidin, NaF, and cycloheximide significantly increased D-factor RNA levels.
    • TNF, TPA, and cycloheximide were shown to stabilize D-factor transcripts, contributing to RNA accumulation.

    Conclusions:

    • D-factor gene expression is regulated by a variety of extracellular stimuli in fibroblasts.
    • D-factor likely participates in the cytokine cascade within mesenchymal cells during inflammatory responses, such as bacterial invasion.
    • The findings highlight D-factor's role in cellular communication and immune responses.