Related Experiment Video
Updated: Jul 18, 2026
10:25
Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
SVEP1 expression is regulated in estrogen-dependent manner
I Shur1, E Zemer-Tov, R Socher
1Department of Cell and Developmental Biology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
Journal of Cellular Physiology
|December 2, 2006
Summary
The study shows that estrogen regulates the SVEP1 protein in bone cells and breast cancer cells. SVEP1 may be a biomarker for understanding how estrogen affects cell interactions in bone microenvironments.
Area of Science:
- Molecular Biology
- Endocrinology
- Oncology
Background:
- SVEP1 protein has domains similar to selectin superfamily molecules.
- Previous studies confirmed SVEP1 expression in osteogenic cells.
- This study investigates the regulation of SVEP1 by 17beta-estradiol (17betaE2).
Purpose of the Study:
- To investigate the role of 17betaE2 in regulating SVEP1 expression in osteoblasts and breast carcinoma cells.
- To explore the potential of SVEP1 as a biomarker in estrogen-modulated cellular microenvironments.
Main Methods:
- In vivo and in vitro studies using sham-operated and ovariectomized (OVX) rats.
- 17betaE2 treatment of cultured osteoblasts and breast carcinoma (BC) cell lines.
- Immunohistochemistry and Fluorescence-Activated Cell Sorting (FACS) for SVEP1 detection.
- Chromatin Immunoprecipitation (ChIP) assay to analyze estrogen receptor (ER) binding to the SVEP1 promoter.
Main Results:
- SVEP1 expression was observed in bone marrow cells of sham-operated rats but not in OVX rats.
- 17betaE2 treatment significantly increased SVEP1 levels in cultured osteoblasts.
- SVEP1 was detected in various breast carcinoma cell lines.
- Estrogen receptor binding to the SVEP1 promoter was affected by 17betaE2 and ICI 182,780, regulating SVEP1 mRNA and protein levels in BC cells.
Conclusions:
- Estrogen plays a crucial role in regulating SVEP1 expression in both bone and breast cancer cells.
- SVEP1 may serve as a valuable biomarker for investigating cellular interactions within estrogen-influenced microenvironments, particularly in bone metastasis.
Related Concept Videos
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
01:23Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
01:45Regulation of Nuclear Protein Sorting
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
01:19Regulation of Hormone Secretion
Regulation of hormone secretion is a finely tuned orchestration driven by various types of stimuli, encompassing neural, humoral, and hormonal signals. Environmental cues instigate neural stimuli, where action potentials traverse nerve fibers to reach their designated targets. An illustrative scenario is the body's response to stress, wherein the sympathetic nervous system releases epinephrine from the adrenal glands, inducing the well-known 'fight or flight' reaction.
Humoral stimuli,...
Humoral stimuli,...
01:24Regulation of Angiogenesis and Blood Supply
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...