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Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

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Updated: Jul 5, 2026

A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping
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Comparative study using scanning electron techniques for imaging of micro-architecture and antigen appearance.

R Socher1, D Benayahu

  • 1Department of Cell and Developmental Biology, Sackler School of Medicine, Tel-Aviv University, Israel.

Journal of Microscopy
|May 1, 2008
PubMed
Summary

Scanning electron microscopy visualized cell surface structures and SVEP1 protein distribution on eukaryotic cells. This technique aids in understanding cell surface antigen appearance and localization for biological research.

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Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Immunolabeling

Background:

  • Eukaryotic cell surface analysis requires advanced imaging techniques.
  • Understanding the distribution of cell surface antigens is crucial in cell biology.
  • Scanning electron microscopy (SEM) offers high resolution for surface topography.

Purpose of the Study:

  • To compare different SEM methods for analyzing eukaryotic cell surface structures.
  • To achieve molecular localization of the SVEP1 protein on cell membranes.
  • To visualize the distribution of SVEP1 using immuno-gold labeling.

Main Methods:

  • Utilized conventional, high-resolution, and environmental SEM for cell surface imaging.
  • Employed immuno-gold labeling with specific antibodies for SVEP1 detection.
  • Integrated cell imaging with molecular localization techniques.

Main Results:

  • SEM methods successfully visualized sub-micrometre surface features on cell membranes.
  • Immuno-gold labeling enabled precise identification of SVEP1 localization.
  • Comparative analysis revealed the effectiveness of different SEM techniques for SVEP1 distribution imaging.

Conclusions:

  • Various SEM technologies are effective for analyzing cell surface structures and antigen appearance.
  • The immuno-gold technique combined with SEM provides detailed molecular imaging of cell surface proteins like SVEP1.
  • This approach enhances the understanding of cell surface antigen distribution in eukaryotic cells.