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Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Published on: February 25, 2007

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Hematopoietic stem cells

Robert G Hawley1, Ali Ramezani, Teresa S Hawley

  • 1Department of Anatomy and Cell Biology, The George Washington University Medical Center, Washington, DC, USA.

Methods in Enzymology
|December 5, 2006
PubMed

Hematopoietic stem cells (HSCs) have the capacity to self-renew and the potential to differentiate into all of the mature blood cell types. The ability to prospectively identify and isolate HSCs has been the subject of extensive investigation since the first transplantation studies implying their existence almost 50 years ago. Despite significant advances in enrichment protocols, the continuous in vitro propagation of human HSCs has not yet been achieved. This chapter describes current procedures used to phenotypically and functionally characterize candidate human HSCs and initial efforts to derive permanent human HSC lines.

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