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Updated: Jul 18, 2026

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
Published on: August 20, 2017
Human blastocyst culture and derivation of embryonic stem cell lines
1Department of Obstetrics and Gynaecology, National University of Singapore, Kent Ridge, Singapore 119074. obgbongs@nus.edu.sg
Abstract:
Human embryonic stem cell (hESC) biology is expected to revolutionize the future of medicine by the provision of cell-based therapies for the treatment of a variety of deliberatig diseases. The tremendous versatility of hESCs has reinforced this hope. To understand the biology of these mysterious cells and attempt to differentiate them into desirable tissues, bona fide hESCs that maintain their stability with time are required for research and clinical application. This review discusses the various protocols to derive and propagate hESCs from high quality embryos. The nature and properties of hESCs are also described together with unanswered questions that need to be addressed if this science is to be taken to the bedside.
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