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Combining site-specific mutagenesis and seeding as a strategy to crystallize 'difficult' proteins: the case of

Goedele Roos1, Elke Brosens, Khadija Wahni

  • 1Brussels Center for Redox Biology, Vlaams Interuniversitair Instituut voor Biotechnologie at the Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussel, Belgium.

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
|December 5, 2006
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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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