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Related Experiment Video

Updated: Jul 18, 2026

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization

Published on: July 27, 2021

Further studies on using multiple-cross mapping (MCM) to map quantitative trait loci.

Barry Malmanger¹, Maureen Lawler, Shannon Coulombe

¹Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, Oregon 97239-3098, USA.

Mammalian Genome : Official Journal of the International Mammalian Genome Society

|December 5, 2006

Summary

Researchers mapped quantitative trait loci (QTLs) for alcohol response in mice. While most QTLs were cross-specific, one on chromosome 1 was consistently found, highlighting the value of multiple-cross mapping (MCM) and heterogeneous stock (HS) analysis for genetic studies.

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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Area of Science:

Genetics
Neuroscience
Pharmacology

Background:

Quantitative trait loci (QTLs) are crucial for understanding complex traits like alcohol response.
The laboratory mouse genome offers a model for genetic mapping due to its relatively simple structure.
Multiple-cross mapping (MCM) is a strategy to identify conserved QTLs across different genetic backgrounds.

Purpose of the Study:

To identify QTLs associated with acute ethanol-induced activation using multiple F(2) intercrosses.
To test the hypothesis that conserved QTLs can be detected across multiple crosses.
To evaluate the utility of heterogeneous stock (HS) animals for high-resolution QTL mapping.

Main Methods:

Whole-genome scans were performed on six F(2) intercrosses derived from C57BL/6J, DBA/2J, BALB/cJ, and LP/J inbred mouse strains.
QTLs were identified based on LOD scores greater than 4.
Fine-mapping of a specific QTL was conducted in HS animals.

Main Results:

QTLs associated with ethanol response were detected on chromosomes 1, 2, 3, 8, 9, 13, 14, and 16.
Conserved QTLs were primarily observed on distal chromosome 1 across multiple crosses.
High-resolution mapping in HS animals localized a chromosome 2 QTL to a 3-Mbp interval with a specific haplotype structure (B6-L:C-D2).

Conclusions:

The study provides evidence that while some QTLs are cross-specific, conserved QTLs can be identified using MCM.
Mapping in HS animals offers enhanced QTL resolution and detailed haplotype information.
This approach advances the understanding of the genetic architecture of alcohol response.