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Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
Published on: May 2, 2018
Plasmid-mediated KPC-2 in a Klebsiella pneumoniae isolate from China
Ze-Qing Wei1, Xiao-Xing Du, Yun-Song Yu
1Key Laboratory of Infectious Diseases of the Public Health Ministry, 1st Affiliated Hospital, Medical School, Zhejiang University, Hangzhou, Zhejiang, China.
Antimicrobial Agents and Chemotherapy
|December 6, 2006
Abstract:
A carbapenem-resistant isolate of Klebsiella pneumoniae producing class A carbapenemase KPC-2 was identified in Zhejiang, China. The KPC-2 gene was located on an approximately 60-kb plasmid in a genetic environment partially different from that of blaKPC-2 in the isolates from the United States and Colombia.
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Plasmids
Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...
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