Related Experiment Video
Updated: Jul 18, 2026
Establishment of an Embryo Implantation Model In Vitro
Published on: June 21, 2024
[Establishment of autologous endometrial coculture and sequential system for human early embryo culture]
Ning-yuan Zhang1, Ya-li Hu, Hai-xiang Sun
1Reproductive Medicine Center, Drum Tower Hospital Affiliated to Nanjing University Medical College, Nanjing, Jiangsu 210008, China.
Objective:
To establish a coculture and a sequential system for human early embryo culture.
Methods:
The endometrial tissue was digested enzymatically and cultured to achieve generated and cryo-thawed endometrial monolayer cells. The generated and cryo-thawed monolayer cells were cocultured with human 2PN embryos and transferred to sequential medium every 48 hours.
Results:
Human endometrial cells had viability in vitro culture. The autologously generated and cryo-thawed monolayer cells were successfully obtained, and 74.04% of the cryo-thawed cells were successfully used in coculturing human early embryos. The embryos developed well, with the clinical pregnancy rate of 68.83% and the implantation rate of 44.23%.
Conclusion:
The autologous endometrial cell coculture and sequential culture system for human early embryo development provides a feasible method for studying human embryo development and implantation so as to improve embryo quality.
