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Purification and characterization of liver arylformamidase in rainbow trout and cattle
1Department of Food Science and Technology, Tokyo University of Fisheries, Japan.
Comparative Biochemistry and Physiology. B, Comparative Biochemistry
|January 11, 1991
Abstract:
1. Arylformamidases were purified from the liver of rainbow trout and cattle. 2. Optimal pH's were 7.7 and 8.0 for the fish and cattle enzyme, respectively. Both enzymes showed optimum temperature at 40 degrees C. Thermal and pH stability ranges and Arrhenius plots of the enzymes differed. 3. Km and molecular weight of the fish enzyme were determined to be 0.28 mM and 32,700, respectively, while those for the cattle enzyme were 0.78 mM and 42,5000, respectively.