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Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
07:42

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Published on: September 19, 2018

TIK, a novel serine/threonine kinase, is recognized by antibodies directed against phosphotyrosine

P L Icely1, P Gros, J J Bergeron

  • 1Department of Medicine, University of Ottawa, Ontario, Canada.

The Journal of Biological Chemistry
|August 25, 1991
PubMed

Insights

Researchers identified a novel serine and threonine kinase, TIK, from murine pre-B cells. This kinase, TIK, lacks typical tyrosine kinase features despite initial antibody screening.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cell Signaling

Background:

  • Protein kinases play crucial roles in cellular signaling pathways.
  • Tyrosine kinases are a significant class of kinases involved in cell growth and differentiation.
  • Identifying novel kinases is essential for understanding complex biological processes.

Purpose of the Study:

  • To isolate and characterize novel kinase enzymes from murine pre-B cells.
  • To identify kinases reactive to anti-phosphotyrosine antibodies.
  • To elucidate the biochemical and sequence characteristics of a newly discovered kinase.

Main Methods:

  • Screening of a lambda gt11 cDNA expression library using anti-phosphotyrosine antibodies.
  • cDNA sequencing and sequence analysis of isolated clones.
  • Biochemical assays to determine kinase activity (serine, threonine, or tyrosine).
  • Analysis of mRNA transcripts in various murine tissues.

Main Results:

  • Isolation of cDNAs encoding kinases, including the known tyrosine kinase c-lyn.
  • Characterization of a novel kinase, designated TIK.
  • Sequence analysis revealed TIK lacks conserved features of protein tyrosine kinases.
  • Biochemical assays demonstrated TIK possesses serine and threonine kinase activity, not tyrosine kinase activity.
  • The TIK protein contains a cdc2 phosphorylation consensus sequence.
  • Three TIK mRNA transcripts were detected in adult murine tissues.

Conclusions:

  • A novel serine and threonine kinase, TIK, has been identified and characterized.
  • TIK represents a new class of kinase, distinct from typical protein tyrosine kinases.
  • The presence of a cdc2 phosphorylation site suggests potential regulation by cell cycle-dependent kinases.
  • TIK is expressed in various adult murine tissues, indicating a potentially widespread biological role.

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