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Updated: Jul 18, 2026

Optimization of Performance Parameters of the TAGGG Telomere Length Assay
Published on: April 21, 2023
Telomere shortening in human HL60 cells by treatment with deoxyguanosine analogs
Toyofumi Yamaguchi1, Hazuki Takahashi, Yoko Harada
1Department of Biological Sciences, Teikyo University of Science and Technology, Uenohara, Yamanashi 409-0193, Japan.
Abstract:
Telomerase is a cellular endogenous reverse transcriptase thought to play an important role in the maintenance of telomere length. We investigated the effects of 3'-azido-2',3'-dideoxyguanosine (AZddG) and carbocyclic oxetanocin G (C.OXT-G), of which the triphosphate derivatives AZddGTP and C.OXT-GTP show potent telomerase inhibition, on telomere length of human HL60 cells in culture. Although AZddG caused more significant telomere shortening than C.OXT-G, only a slight decrease of cell growth rate was observed.
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