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Published on: June 30, 2023
A novel convenient method for high bacteriophage titer assay
Kazuo Yoshida1, Yuki Nasu, Nozomi Shitami
1Department of Nutritional Sciences, Yasuda Women's University, Hiroshima 731-0153, Japan. kazuyosi@hiroshima-u.ac.jp
Nucleic Acids Symposium Series (2004)
|September 15, 2009
Summary
Phage titer assays are crucial for bacteriophage research. A new cross-streak and paper disk method simplifies high-titer concentration determination, avoiding tedious plaque counting.
Area of Science:
- Microbiology
- Virology
- Bacteriophage Research
Background:
- Bacteriophages (phages) are gaining attention for applications like phage therapy.
- Accurate phage titer assays are essential for phage research and applications.
- Conventional plaque counting methods are laborious and unsuitable for high phage titers.
Purpose of the Study:
- To develop a novel, convenient method for determining high bacteriophage titers.
- To overcome the limitations of traditional plaque counting assays.
Main Methods:
- A new
- cross streak and paper disk assay method
- was developed and described.
Main Results:
- The described method allows for high phage titer concentration determination.
- This approach eliminates the need for plaque counting.
Conclusions:
- The novel cross-streak and paper disk assay offers a convenient alternative for high-titer bacteriophage quantification.
- This method simplifies phage experiments and expands their applicability to higher concentrations.
