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A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
Published on: October 21, 2010
[Disk diffusion method for fluconazole susceptibility testing of Candida spp. isolates]
1Departamento Micología, INEI ANLIS Dr. Carlos G. Malbran, Avenida Vélez Sarsfield 563 (C1282AFF) Ciudad Autónoma de Buenos Aires, Argentina.
Revista Argentina De Microbiologia
|December 13, 2006
Summary
A standardized disk diffusion method accurately detects fluconazole susceptibility in yeast isolates, offering a reliable alternative to microdilution. This cost-effective method shows high agreement for antifungal drug susceptibility testing.
Area of Science:
- Medical Mycology
- Clinical Microbiology
- Antimicrobial Susceptibility Testing
Background:
- Accurate in vitro antifungal susceptibility testing is crucial for guiding treatment of yeast infections.
- Standardization of methods like disk diffusion is needed for reliable fluconazole susceptibility detection.
Purpose of the Study:
- To standardize and evaluate a visual disk diffusion method for detecting in vitro fluconazole susceptibility in clinical yeast isolates.
- To compare the disk diffusion method with the CLSI M27-A2 broth microdilution reference method.
Main Methods:
- Tested 1193 clinical yeast isolates, including various Candida species.
- Compared disk diffusion results with MICs from a modified EUCAST broth microdilution method.
- Established interpretative breakpoints for zone diameters and fluconazole MICs.
Main Results:
- Overall agreement between disk diffusion and microdilution was 94.7%.
- Very major and major error rates were low (0.2% and 0.3%, respectively).
- Good inter- and intralaboratory agreement was observed, indicating reproducibility.
Conclusions:
- The disk diffusion method is an inexpensive, reliable, and reproducible technique for Candida spp. drug susceptibility testing.
- It is advisable to confirm results with the reference microdilution method when inhibition zones are ≤15 mm.
