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[Disk diffusion method for fluconazole susceptibility testing of Candida spp. isolates].

L Rodero¹, S Córdoba, W Vivot

¹Departamento Micología, INEI ANLIS Dr. Carlos G. Malbran, Avenida Vélez Sarsfield 563 (C1282AFF) Ciudad Autónoma de Buenos Aires, Argentina.

Revista Argentina De Microbiologia

|December 13, 2006

Summary

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A standardized disk diffusion method accurately detects fluconazole susceptibility in yeast isolates, offering a reliable alternative to microdilution. This cost-effective method shows high agreement for antifungal drug susceptibility testing.

Area of Science:

Medical Mycology
Clinical Microbiology
Antimicrobial Susceptibility Testing

Background:

Accurate in vitro antifungal susceptibility testing is crucial for guiding treatment of yeast infections.
Standardization of methods like disk diffusion is needed for reliable fluconazole susceptibility detection.

Purpose of the Study:

To standardize and evaluate a visual disk diffusion method for detecting in vitro fluconazole susceptibility in clinical yeast isolates.
To compare the disk diffusion method with the CLSI M27-A2 broth microdilution reference method.

Main Methods:

Tested 1193 clinical yeast isolates, including various Candida species.
Compared disk diffusion results with MICs from a modified EUCAST broth microdilution method.
Established interpretative breakpoints for zone diameters and fluconazole MICs.

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Main Results:

Overall agreement between disk diffusion and microdilution was 94.7%.
Very major and major error rates were low (0.2% and 0.3%, respectively).
Good inter- and intralaboratory agreement was observed, indicating reproducibility.

Conclusions:

The disk diffusion method is an inexpensive, reliable, and reproducible technique for Candida spp. drug susceptibility testing.
It is advisable to confirm results with the reference microdilution method when inhibition zones are ≤15 mm.