[Analysis of factors affecting the thermal stability of alpha 2-macroglobulin in solution]

The factors affecting the thermal stability of alpha 2-macroglobulin (alpha 2M) in aqueous solution have been analyzed with an experiment of orthogonal design. The optimum conditions for heat treatment of alpha 2M have been established. Under these conditions, the trypsin-binding activity of alpha 2M can be fully retained after heat treatment at 60 degrees C for 10 hours.