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The mRNAs encoding acidic FGF, basic FGF and FGF receptor are coordinately downregulated during myogenic
1Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.
Development (Cambridge, England)
|March 1, 1991
Summary
Endogenous acidic and basic fibroblast growth factors (FGFs) and their receptors are downregulated during skeletal muscle cell differentiation. This coordinated decrease may regulate myogenesis, as FGF transcripts decline while myogenin expression increases.
Area of Science:
- Cellular and Molecular Biology
- Muscle Physiology
- Developmental Biology
Background:
- Fibroblast growth factors (FGFs) influence various cell types, including skeletal myocytes.
- Exogenous FGFs promote myoblast proliferation and inhibit differentiation, but endogenous FGF expression in skeletal myocytes is poorly understood.
Purpose of the Study:
- To investigate the endogenous expression of acidic and basic FGFs and their receptor in skeletal myocytes.
- To determine if FGF expression is regulated during myogenic differentiation.
Main Methods:
- Analysis of acidic and basic FGF mRNA expression in murine and rat skeletal muscle.
- Examination of FGF mRNA and FGF receptor mRNA levels in myogenic cell lines (Sol 8 and L6) during differentiation.
- Quantitative assessment of gene expression changes.
Main Results:
- Acidic and basic FGF mRNAs are expressed in skeletal muscle, with variations across tissues and species.
- Both acidic and basic FGF mRNAs are downregulated at the transcriptional level during myogenic differentiation in Sol 8 and L6 cells.
- FGF receptor mRNA levels decrease in parallel with FGF mRNAs during differentiation in Sol 8 cells.
Conclusions:
- A coordinated downregulation of endogenous FGFs and their receptor may play a role in regulating myogenic differentiation.
- Declining FGF expression correlates with increasing myogenin expression, suggesting a mechanism for FGFs in differentiation control.