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Updated: Aug 11, 2026

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
Published on: August 13, 2013
T-cell regulation of CD5+ B-cell activity in normal mice
R Huchet1, D Grandjon, O Halle-Pannenko
1Department of Immunogenetics, Paul Brousse Hospital, Villejuif, France.
Abstract:
The differentiation of plaque-forming cell (PFC) precursors against bromelain-treated syngeneic erythrocytes (Br MRBC) into PFC induced in vitro by LPS is down-regulated by nylon non-adherent (nylon-passed--NP) T cells and by nylon adherent (NA) T cells. NA T cells are more potent inhibitors than NP T cells. This regulatory activity of NA and NP T cells results from an interaction between CD4+ radioresistant and CD8+ radiosensitive T cells. Furthermore CD4+ T cells from the NA fraction but not from the NP fraction are activated cells: their inhibitory activity is abrogated after preincubation with cycloheximide. These results are discussed within the overall framework of T-cell regulation of autoimmune anti-Br MRBC B-cell subsets.
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