Related Experiment Video
Updated: Jul 18, 2026

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
Published on: August 22, 2019
EMBASE search strategies achieved high sensitivity and specificity for retrieving methodologically sound systematic
Nancy L Wilczynski1, R Brian Haynes,
1Health Information Research Unit, Department of Clinical Epidemiology and Biostatistics, McMaster University, Hamilton, Ontario, Canada. wilczyn@mcmaster.ca
Objectives:
Systematic reviews of the literature are instrumental for bridging research to health care practice and are widely available through databases such as MEDLINE and EMBASE. Search strategies have been developed to aid users in MEDLINE, but no empirical work has been done for EMBASE. The objective of this study was to develop search strategies that optimize the retrieval of methodologically sound systematic reviews from EMBASE.
Study Design And Setting:
An analytic survey was conducted, comparing hand searches of 55 journals with retrievals from EMBASE for 4,843 candidate search terms and 17,004 combinations. Candidate search strategies were run in EMBASE, the retrievals being compared with the hand search data. The sensitivity, specificity, precision, and accuracy of the search strategies were calculated.
Results:
Two hundred twenty (16.2%) of the 1,354 articles classified as a review met basic criteria for scientific merit. Combinations of search terms reached peak sensitivities of 94.6% with specificity at 63.7%, whereas combinations of search terms to optimize specificity reached peak specificities of 99.3% with sensitivity at 61.4%.
Conclusion:
Empirically derived search strategies can achieve high sensitivity and specificity for retrieving methodologically sound systematic reviews from EMBASE.
Related Concept Videos
Dose-Response Relationship: Selectivity and Specificity
Enzyme-Linked Immunosorbent Assay
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast, controlled...