Related Experiment Video
Updated: Jul 18, 2026
Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells
Published on: June 2, 2023
Peroxiredoxin I and II inhibit H2O2-induced cell death in MCF-7 cell lines
Ji-Yeon Bae1, Soo-Jung Ahn, Wonshik Han
1Cancer Research Institute, Seoul National University, Jongno-Gu, Seoul, Korea.
Abstract:
Apoptosis is known to be induced by direct oxidative damage due to oxygen-free radicals or hydrogen peroxide or by their generation in cells by the actions of injurious agents. Together with glutathione peroxidase and catalase, peroxiredoxin (Prx) enzymes play an important role in eliminating peroxides generated during metabolism. We investigated the role of Prx enzymes during cellular response to oxidative stress. Using Prx isoforms-specific antibodies, we investigated the presence of Prx isoforms by immunoblot analysis in cell lysates of the MCF-7 breast cancer cell line. Treatment of MCF-7 with hydrogen peroxide (H2O2) resulted in the dose-dependent expressions of Prx I and II at the protein and mRNA levels. To investigate the physiologic relevance of the Prx I and II expressions induced by H2O2, we compared the survivals of MCF10A normal breast cell line and MCF-7 breast cancer cell line following exposure to H2O2. The treatment of MCF10A with H2O2 resulted in rapid cell death, whereas MCF-7 was resistant to H2O2. In addition, we found that Prx I and II transfection enabled MCF10A cells to resist H2O2-induced cell death. These findings suggest that Prx I and II have important functions as inhibitors of cell death during cellular response to oxidative stress.
Insights
Peroxiredoxin (Prx) I and II enzymes protect cells from oxidative stress. Overexpression of Prx I and II in normal breast cells enhanced their resistance to hydrogen peroxide-induced cell death.
Area of Science:
- Biochemistry
- Cell Biology
- Molecular Biology
Background:
- Oxidative stress, caused by reactive oxygen species like hydrogen peroxide (H2O2), can induce apoptosis.
- Peroxiredoxin (Prx) enzymes are crucial for detoxifying peroxides generated during cellular metabolism.
- Understanding the role of Prx enzymes in cellular defense against oxidative damage is vital.
Purpose of the Study:
- To investigate the role of Prx enzymes in the cellular response to oxidative stress.
- To determine the expression patterns of Prx isoforms in breast cancer cells under oxidative conditions.
- To assess the protective function of Prx I and II against H2O2-induced cell death.
Main Methods:
- Immunoblot analysis using Prx isoform-specific antibodies to detect Prx presence in MCF-7 cell lysates.
- Quantitative analysis of Prx I and II protein and mRNA expression following H2O2 treatment.
- Comparative survival assays of MCF10A and MCF-7 cell lines exposed to H2O2.
- Transfection studies to evaluate the impact of Prx I and II expression on cell survival.
Main Results:
- Hydrogen peroxide (H2O2) treatment induced dose-dependent expression of Prx I and II in MCF-7 breast cancer cells at both protein and mRNA levels.
- MCF-7 cells exhibited resistance to H2O2-induced cell death, while normal MCF10A cells underwent rapid cell death.
- Transfection of Prx I and II into MCF10A cells conferred resistance to H2O2-induced apoptosis.
Conclusions:
- Prx I and II play a significant role in protecting cells against oxidative stress.
- The enhanced expression of Prx I and II contributes to the resistance of MCF-7 breast cancer cells to H2O2.
- Prx I and II function as critical inhibitors of cell death during the cellular response to oxidative damage.
More Related Videos
08:57Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
04:53Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
Related Concept Videos
01:24Peroxisomes
01:30Peroxisomes
01:46Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
01:17Oxidation of Phenols to Quinones
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox property is crucial in...
01:30The Electron Transport Chain
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q in...
01:16Necrosis
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become anucleated and die, but their...