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Updated: Jul 18, 2026
Optical Clearing and Imaging of Immunolabeled Kidney Tissue
Published on: July 22, 2019
Effect of optical clearing agents on the in vivo optical properties of squamous epithelial tissue
Stacy R Millon1, Katherine M Roldan-Perez, Kristin M Riching
1Department of Biomedical Engineering, University of Wisconsin, Madison, Wisconsin 53706, USA.
Background And Objectives:
Optical clearing agents (OCAs) have previously been shown to increase depth penetration within turbid tissue ex vivo. This paper quantifies tissue optical properties of the hamster cheek pouch model in order to provide a means to assess the effect of OCAs quantitatively in vivo.
Study Design/Materials And Methods:
Diffuse reflectance spectra were obtained from both cheeks of 12 hamsters before and after immersion in dimethyl sulfoxide (DMSO), glycerol or a phosphate buffer saline (PBS) control for 20 minutes. A Monte Carlo model was then utilized to derive the wavelength dependent reduced scattering and absorption coefficients.
Results:
DMSO caused a statistically significant decrease in the absorption and reduced scattering coefficients derived by the model. Glycerol caused a statistically significant increase in the wavelength dependent absorption coefficient, but no statistically significant changes in the reduced scattering coefficient.
Conclusions:
DMSO and glycerol act upon tissues differently as reflected by the tissue optical properties, implying that not all OCAs are equally effective in optically clearing tissues.
