Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Behavior00:54

Electron Behavior

Electrons are negatively charged subatomic particles that are attracted to an orbit around the positively-charged nucleus of an atom. They reside in locations that are associated with energy levels called shells and are further organized into sub-shells and orbitals within each shell.Electrons Orbit the NucleusElectrons are found in specific locations outside of the nucleus. The shell in which an electron resides indicates the general energy level of the electron: those closer to the nucleus...
Resting Membrane Potential01:24

Resting Membrane Potential

The relative difference in electrical charge, or voltage, between the inside and the outside of a cell membrane, is called the membrane potential. It is generated by differences in permeability of the membrane to various ions and the concentrations of these ions across the membrane.
The Inside of a Neuron is More Negative
The membrane potential of a cell can be measured by inserting a microelectrode into a cell and comparing the charge to a reference electrode in the extracellular fluid. The...
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Induced Electric Dipoles01:28

Induced Electric Dipoles

A permanent electric dipole orients itself along an external electric field. This rotation can be quantified by defining the potential energy because the external torque does work in rotating it. Then, the potential energy is minimum at the parallel configuration and maximum at the antiparallel configuration. While the former is a stable equilibrium, the latter is an unstable equilibrium.
Since the absolute value of potential energy holds no physical meaning, its zero value can be chosen as per...
Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Electrochemical Systems01:24

Electrochemical Systems

Electrochemical systems provide a fascinating insight into the dynamic interplay of charged species within various phases. One notable example is the interaction between a membrane permeable to K⁺ ions but not to Cl⁻ ions, separating an aqueous KCl solution from pure water. As K⁺ ions diffuse through the membrane, they generate net charges on each phase, leading to a potential difference between them.Similarly, when a piece of Zn is immersed in an aqueous ZnSO₄ solution, the Zn metal, composed...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Simulating Metal-Imidazole Complexes.

Journal of chemical theory and computation·2024
Same author

Role of global public sector research in discovering new drugs and vaccines.

The Journal of technology transfer·2023
Same author

Protein oxidation involved in Cys-Tyr post-translational modification.

Journal of inorganic biochemistry·2017
Same author

Wide dynamic range sensing with single quantum dot biosensors.

ACS nano·2012
Same author

Identifying proteins that can form tyrosine-cysteine crosslinks.

Metallomics : integrated biometal science·2012
Same author

Submicron hard X-ray fluorescence imaging of synthetic elements.

Analytica chimica acta·2012

Related Experiment Video

Updated: Jul 8, 2026

Fabrication of Carbon Nanotube High-Frequency Nanoelectronic Biosensor for Sensing in High Ionic Strength Solutions
12:20

Fabrication of Carbon Nanotube High-Frequency Nanoelectronic Biosensor for Sensing in High Ionic Strength Solutions

Published on: July 22, 2013

Electron donor solvent effects provide biosensing with quantum dots.

Baikuntha P Aryal1, David E Benson

  • 1Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA.

Journal of the American Chemical Society
|December 15, 2006
PubMed
Summary
This summary is machine-generated.

A novel palmitate biosensor utilizes semiconducting nanoparticles and electron transfer to detect palmitate concentration. This method offers a new approach for developing fluorescence imaging reagents for various small molecules.

More Related Videos

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

Electric Cell-Substrate Sensing for Real-Time Evaluation of Metal-Organic Framework Toxicological Profiles
04:53

Electric Cell-Substrate Sensing for Real-Time Evaluation of Metal-Organic Framework Toxicological Profiles

Published on: May 26, 2023

Related Experiment Videos

Last Updated: Jul 8, 2026

Fabrication of Carbon Nanotube High-Frequency Nanoelectronic Biosensor for Sensing in High Ionic Strength Solutions
12:20

Fabrication of Carbon Nanotube High-Frequency Nanoelectronic Biosensor for Sensing in High Ionic Strength Solutions

Published on: July 22, 2013

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

Electric Cell-Substrate Sensing for Real-Time Evaluation of Metal-Organic Framework Toxicological Profiles
04:53

Electric Cell-Substrate Sensing for Real-Time Evaluation of Metal-Organic Framework Toxicological Profiles

Published on: May 26, 2023

Area of Science:

  • Biochemistry
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Biosensors are crucial for detecting small molecules.
  • Semiconducting nanoparticles offer unique optical properties for sensing applications.
  • Intestinal fatty acid binding protein (IFABP) plays a role in fatty acid transport.

Purpose of the Study:

  • To develop a novel biosensor for sensitive palmitate detection.
  • To utilize the fluorescence of semiconducting nanoparticles for reporting palmitate concentration.
  • To explore the use of IFABP in modulating biosensor properties.

Main Methods:

  • Constructed a biosensor using ZnS-coated CdSe nanoparticles.
  • Covalently attached a ruthenium complex-modified IFABP to the nanoparticles.
  • Utilized electron transfer quenching of nanoparticle emission to detect palmitate.
  • Measured changes in fluorescence intensity upon palmitate addition.

Main Results:

  • Observed a 1.6-fold change in emission intensity with 500 nM sodium palmitate.
  • Determined a dissociation constant of 5 nM for palmitate.
  • Established a lower limit of detection at 1 nM.
  • Demonstrated that palmitate binding alters pocket solvation without changing global protein conformation.

Conclusions:

  • Developed a new class of biosensors based on semiconducting nanoparticles.
  • The biosensor exhibits high sensitivity and a low detection limit for palmitate.
  • This strategy can be extended to detect thousands of other small molecule analytes.
  • The biosensors can serve as fluorescence contrast imaging reagents.