Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

IFN-gamma and TNF-alpha decrease serotonin transporter function and expression in Caco2 cells.

Kevin F Foley1, Cristen Pantano, Allison Ciolino

  • 1Department of Medical Laboratory and Radiation Sciences, the University of Vermont, Burlington, VT 05405, USA.

American Journal of Physiology. Gastrointestinal and Liver Physiology
|December 16, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fundamentals of Neurogastroenterology: Basic Science.

Gastroenterology·2026
Same author

Microbial dysbiosis alters serotonin signaling in a postinflammatory murine model of visceral pain.

American journal of physiology. Gastrointestinal and liver physiology·2025
Same author

Primary secretory carcinoma of thyroid with unusual features mimicking metastasis?

Diagnostic pathology·2025
Same author

Early life exposure to broccoli sprouts confers stronger protection against enterocolitis development in an immunological mouse model of inflammatory bowel disease.

mSystems·2023
Same author

Protective actions of a luminally acting 5-HT<sub>4</sub> receptor agonist in mouse models of colitis.

Neurogastroenterology and motility·2023
Same author

Steamed broccoli sprouts alleviate DSS-induced inflammation and retain gut microbial biogeography in mice.

mSystems·2023

Inflammatory cytokines like TNF-alpha and IFN-gamma reduce serotonin transporter (SERT) function and expression in gut epithelial cells. This finding may explain altered gut function in inflammatory bowel diseases.

Area of Science:

  • Gastroenterology
  • Neuroscience
  • Immunology

Background:

  • Decreased serotonin transporter (SERT) expression is observed in colitis, ulcerative colitis, and irritable bowel syndrome.
  • Altered SERT function may contribute to motility, secretion, and sensation changes in these gut disorders.

Purpose of the Study:

  • To investigate the role of inflammatory mediators in downregulating colonic SERT expression.
  • To identify specific cytokines that affect SERT function in cultured colonic cells.

Main Methods:

  • Cultured Caco2 cells were treated with conditioned medium from activated human lymphocytes.
  • Cells were exposed to individual proinflammatory agents, including TNF-alpha and IFN-gamma.
  • Serotonin uptake, SERT mRNA, and protein levels were measured.

Related Experiment Videos

Main Results:

  • Tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) significantly decreased SERT function after 72-hour incubation.
  • These cytokines also reduced SERT mRNA and protein levels without causing cytotoxicity.
  • Prostaglandin E2 (PGE2), IL-12, and S-nitrosoglutathione (GSNO) had different effects on SERT uptake.

Conclusions:

  • TNF-alpha and IFN-gamma can downregulate SERT expression in colonic epithelial cells.
  • These cytokines may contribute to the altered gut motility and sensation observed in inflammatory bowel conditions like ulcerative colitis and irritable bowel syndrome.