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Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
Published on: May 18, 2018
Terminal complement complex C5b-9-treated human monocyte-derived dendritic cells undergo maturation and induce Th1
Yongwen Chen1, Chengying Yang, Naishi Jin
1Laboratory of Immunoregulation, Institute of Immunology, PLA, The Third Military Medical University, Chongqing, P R China.
European Journal of Immunology
|December 16, 2006
Summary
Sublytic complement C5b-9 activates dendritic cells (DC), promoting their maturation and enhancing T cell responses. This suggests C5b-9 bridges innate and acquired immunity by regulating DC function.
Area of Science:
- Immunology
- Complement system
- Cellular immunology
Background:
- Sublytic C5b-9 is a pro-inflammatory mediator.
- Dendritic cells (DC) are crucial for immune responses.
- The role of C5b-9 in DC function is largely unknown.
Purpose of the Study:
- To investigate the effect of sublytic C5b-9 on dendritic cell (DC) maturation and function.
- To determine if C5b-9 influences DC-mediated T cell activation.
Main Methods:
- In vitro assembly of functional C5b-9 using purified complement proteins.
- Analysis of DC maturation markers (CD83, HLA, costimulatory molecules).
- Measurement of cytokine secretion (IL-12, TNF-alpha) and antigen uptake.
- Mixed lymphocyte reactions to assess DC-T cell activation and polarization.
Main Results:
- Sublytic C5b-9 significantly promoted DC maturation, indicated by increased CD83, HLA-antigens, and costimulatory molecules (CD80, D86, B7-H1, B7-H3, B7-H4, BTLA).
- C5b-9-treated DCs showed increased secretion of IL-12 and TNF-alpha, with reduced antigen uptake capacity.
- C5b-9-activated DCs effectively stimulated CD4+ T cell activation, promoting interferon-gamma and IL-2 production.
- DCs treated with C5b-9 induced Th1 polarization in naive CD4+ CD45RA+ T cells.
Conclusions:
- Sublytic C5b-9 induces dendritic cell maturation and enhances their immunoregulatory capacity.
- C5b-9 acts as a bridge between innate and acquired immunity by modulating DC function.
- DCs are identified as potential immunoregulatory targets of the complement system's C5b-9 component.
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