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Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
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Marker-exchange mutagenesis and complementation strategies for the Gram-negative bacteria Xanthomonas oryzae pv.
Sang-Won Lee1, Pamela C Ronald
1Department of Plant Pathology, University of California, Davis, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 19, 2006
Abstract:
This chapter describes methods for targeted knockouts using marker exchange mutagenesis and complementation of the Gram-negative bacteria Xanthomonas oryzae pv. oryzae. We have used these methods to demonstrate that type I secretion and modification systems are involved in avrXa21 activity of X. oryzae pv. oryzae.
