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Updated: Jul 18, 2026
14:57
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
TIS11D is a candidate pro-apoptotic p53 target gene
Roger S Jackson1, Yong-Jig Cho, Peng Liang
1Vanderbilt-Ingram Comprehensive Cancer Center, Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, Tennessee 37232-6840, USA.
Cell Cycle (Georgetown, Tex.)
|December 19, 2006
Summary
Researchers identified TIS11D as a gene induced by the tumor suppressor p53. This TIS11D gene induction inhibited cell proliferation and promoted apoptosis, suggesting its role in p53-mediated cell death.
Area of Science:
- Molecular Biology
- Cancer Research
- Cell Biology
Background:
- The tumor suppressor p53 plays a critical role in regulating cellular responses to stress, including apoptosis.
- While many p53 target genes are known, the precise mechanisms governing p53-dependent apoptosis are not fully understood.
Purpose of the Study:
- To identify novel p53 target genes involved in apoptosis.
- To investigate the functional role of TIS11D in p53-mediated cellular processes.
Main Methods:
- Comprehensive screening for p53 target genes using differential display.
- Confirmation of TIS11D gene induction via Northern Blot analysis.
- Assessment of cell proliferation and apoptosis following TIS11D expression.
Main Results:
- TIS11D was identified as a novel gene induced by p53.
- Northern Blot confirmed the induction of TIS11D mRNA in response to p53.
- Inducible TIS11D expression led to significant inhibition of cell proliferation and induced apoptosis.
Conclusions:
- TIS11D is a candidate p53 target gene.
- TIS11D may be a key component of the molecular network mediating p53-dependent apoptosis.
- Further research into TIS11D function can elucidate p53's role in cancer suppression.
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