Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chemical methods for glycoprotein discovery.

Michelle R Bond1, Jennifer J Kohler

  • 1Department of Chemistry, Stanford University, Keck Science Building, Stanford, CA 94305, USA.

Current Opinion in Chemical Biology
|December 19, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

B3GNT7 regulates mucin glycosylation and protects against colitis and infection.

bioRxiv : the preprint server for biology·2026
Same author

Hypofucosylation promotes pertussis toxin binding to cell surface glycococonjugates and pertussis toxin-induced intracellular ERK signaling.

Glycobiology·2026
Same author

Hypofucosylation promotes pertussis toxin binding to cell surface glycococonjugates and pertussis toxin-induced intracellular ERK signaling.

bioRxiv : the preprint server for biology·2026
Same author

Time-Resolved Tracking and Secretome Mapping of Intracellular, Cell Surface, and Extracellular Glycoconjugates with a Mini-Tetrazine N-Acetylglucosamine.

bioRxiv : the preprint server for biology·2025
Same author

CD33-CD45 Interaction Reveals a Mechanistic Link to Alzheimer's Disease Susceptibility.

bioRxiv : the preprint server for biology·2025
Same author

Fucosylation of glycoproteins and glycolipids: opposing roles in cholera intoxication.

Nature chemical biology·2024
Same journal

Function through shape: An overview of DNA G-quadruplexes in transcriptional regulation.

Current opinion in chemical biology·2026
Same journal

Advances in tools and technologies for multiplexed bioluminescence imaging.

Current opinion in chemical biology·2026
Same journal

High-resolution molecular mapping by expansion-coupled label-free and multimodal imaging.

Current opinion in chemical biology·2026
Same journal

Recent advances in glycoconjugate-based therapeutics.

Current opinion in chemical biology·2026
Same journal

Towards better red emitters for bioimaging: Innovations in rhodamine and cyanine chemistry.

Current opinion in chemical biology·2026
Same journal

Chemigenetic fluorescent biosensors in biological imaging - New trends and advances.

Current opinion in chemical biology·2026
See all related articles

Researchers are advancing glycoproteomics by developing new methods for isolating and identifying glycosylated proteins. These techniques combine chemical tagging and isotope labeling for precise analysis, aiding biomarker discovery in cancer and developmental biology.

Area of Science:

  • Biochemistry
  • Proteomics
  • Glycobiology

Background:

  • Glycoprotein identification is crucial for understanding biological processes.
  • Current methods for isolating glycosylated proteins from the proteome are limited.
  • Post-translational glycan modifications play significant roles in cellular functions.

Purpose of the Study:

  • To develop and refine methods for the isolation and identification of glycoproteins.
  • To enable accurate characterization of glycosylation sites and modification levels.
  • To facilitate biomarker discovery and functional analysis in glycoproteomics.

Main Methods:

  • Utilizing enzymatic and metabolic methods for chemical tagging of proteins.
  • Employing mass spectrometry for glycoprotein identification.

Related Experiment Videos

  • Incorporating isotope labels via enzymatic or chemoselective reactions for site-specific labeling.

    • Main Results:

    • Successful isolation of glycosylated proteins from complex proteomes.
    • Mass spectrometry-based confirmation of glycoprotein identity and glycosylation sites.
    • Quantification of glycan modification extent using isotopic labeling.

    Conclusions:

    • Combined chemical tagging and isotope labeling offer powerful strategies for glycoproteomics.
    • These techniques are instrumental for identifying disease biomarkers and understanding developmental changes.
    • Future advancements will enable functional glycoproteomic analyses, including interaction partner discovery.