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Antimicrobial polypeptide multilayer nanocoatings.

Jai S Rudra1, Komal Dave, Donald T Haynie

  • 1Biomedical Engineering, Bionanosystems Engineering Laboratory, Center for Applied Physics Studies, PO Box 10348, Louisiana Tech University, Ruston, LA 71272, USA.

Journal of Biomaterials Science. Polymer Edition
|December 21, 2006
PubMed
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Researchers created antimicrobial polypeptide multilayer nanofilms using layer-by-layer assembly (LBL) with hen egg white lysozyme (HEWL). These films effectively inhibit microbial growth, showing promise for food preservation and medical coatings.

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Food Science

Background:

  • Layer-by-layer (LBL) assembly enables fabrication of nanometer-thick multilayer films under mild conditions.
  • Polypeptide multilayer nanofilms can incorporate functional agents, such as antimicrobial proteins.
  • Hen egg white lysozyme (HEWL) is a known antimicrobial agent used as a food preservative.

Purpose of the Study:

  • To assess the feasibility of preparing polypeptide multilayer nanofilms with antimicrobial properties.
  • To incorporate hen egg white lysozyme (HEWL) into nanofilms using LBL assembly.
  • To evaluate the antimicrobial efficacy of these HEWL-containing nanofilms.

Main Methods:

  • Fabrication of multilayer nanofilms using sequential adsorption of poly(L-glutamic acid) (PLGA) and HEWL.

Related Experiment Videos

  • Utilizing LBL assembly under mildly acidic pH conditions.
  • Testing the antimicrobial activity of the PLGA/HEWL nanofilms against Microccocus luteus.

    • Main Results:

    • PLGA/HEWL nanofilms demonstrated inhibition of Microccocus luteus growth.
    • The amount of HEWL released from the films correlated with the number of HEWL layers.
    • Antimicrobial activity was achieved by direct film fabrication on surfaces.

    Conclusions:

    • Polypeptide multilayer nanofilms incorporating HEWL exhibit specific antimicrobial properties.
    • LBL assembly offers a versatile platform for developing functional antimicrobial coatings.
    • Potential applications include food preservation and antimicrobial coatings for medical implants.