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Updated: Jul 18, 2026

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
Published on: July 9, 2012
Comparison of three bacterial detection methods under routine conditions.
M Schmidt1, A Karakassopoulos, J Burkhart
1German Red Cross Institute Frankfurt, Frankfurt, Germany. m.schmidt@blutspende.de
BacT/ALERT detected more bacterial contamination in platelets than Pall eBDS or Scansystem. However, BacT/ALERT had more false positives, while Pall eBDS and Scansystem offered higher specificity for bacterial detection systems.
Area of Science:
- Transfusion Medicine
- Microbiology
- Medical Device Technology
Background:
- Bacterial screening of platelets is mandated in the USA and voluntary in Europe.
- Prevalence of bacterial contamination in platelet concentrates is a critical safety concern.
- This study evaluated bacterial detection systems for platelet concentrates.
Purpose of the Study:
- To investigate the prevalence of bacterially contaminated platelet concentrates.
- To compare the performance of three different bacterial detection systems: BacT/ALERT, Scansystem, and Pall eBDS.
- To assess sensitivity and specificity of each system in detecting bacterial contamination.
Main Methods:
- Prospective multicentre study involving German Red Cross blood donor services.
- Pool platelet concentrates (n=6307) tested with BacT/ALERT, Scansystem, and Pall eBDS.
- Apheresis platelets (n=4730) tested with BacT/ALERT and Pall eBDS.
- Positive results confirmed by a microbiology reference laboratory.
Main Results:
- BacT/ALERT detected one contaminated pool platelet concentrate (1:6307), which Pall eBDS and Scansystem missed.
- Pall eBDS and Scansystem showed higher specificity with fewer false positives compared to BacT/ALERT.
- BacT/ALERT had a false-positive rate of 0.25%, while Pall eBDS had 0.03% and Scansystem 0.0%.
Conclusions:
- BacT/ALERT demonstrated superior sensitivity in detecting bacterial contamination in platelets.
- Pall eBDS and Scansystem exhibited enhanced specificity, reducing false-positive results.
- BacT/ALERT and Pall eBDS are user-friendly, while Scansystem requires specialized training.
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