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[Differential proteomic expression in human liver cells stimulated by hydroquinone]
Li Ju1, Shu-Zhi Zhang, Ran Zhao
1Labour Health and Environmental Hygiene Institute, Zhejiang University, Hangzhou 310031, China.
Objective:
To explore the differential proteomic expression in human liver cells L-02 after exposure to HQ.
Methods:
Subcultured L-02 cells were treated by HQ for 24 h at a 1 x 10(-4) mol/L concentration and a blank group was set as the control. Immediately after the treatment, total cellular proteins were extracted and separated by 2-DE, and the images were analyzed by PDQuest software. The experiment was totally repeated 3 times with 3 repetitions for each group every time. The well repeated spots were identified by MALDI-TOF MS and then searched in NCBI human protein database with Mascot.
Results:
About 1,000 spots per gel were found. Compared with the control group, 17, 18 and 24 spots were significantly altered in 3 separate experiments. The 4 well repeated spots were identified by MALDI-TOF MS as Rho GDP dissociation inhibitor GDI alpha, 6-phosphogluconolactonase, erbB3 binding protein EBP1 and lamin A/C, isoform 1 precursor. They were involved in cell skeleton, signal transduction and energy metabolization in functional classification.
Conclusion:
Hydroquinone can change the protein expression in liver cells, which provides clues for exploring the toxic mechanism.
