Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pyrosequencing: a simple method for accurate genotyping.

Cristi R King1, Tiffany Scott-Horton

  • 1Division of Oncology, Washington University School of Medicine, St. Louis, MO, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 23, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Warfarin Dosing in Patients With CYP2C9*5 Variant Alleles.

Clinical pharmacology and therapeutics·2022
Same author

Cost-Effectiveness Analysis of a Pharmacist-Led Medication Therapy Management Program: Hypertension Management.

Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research·2021
Same author

Patient-derived small intestinal myofibroblasts direct perfused, physiologically responsive capillary development in a microfluidic Gut-on-a-Chip Model.

Scientific reports·2020
Same author

Effect of Low-Intensity vs Standard-Intensity Warfarin Prophylaxis on Venous Thromboembolism or Death Among Patients Undergoing Hip or Knee Arthroplasty: A Randomized Clinical Trial.

JAMA·2019
Same author

Effect of Genotype-Guided Warfarin Dosing on Clinical Events and Anticoagulation Control Among Patients Undergoing Hip or Knee Arthroplasty: The GIFT Randomized Clinical Trial.

JAMA·2017
Same author

Differential β3 Integrin Expression Regulates the Response of Human Lung and Cardiac Fibroblasts to Extracellular Matrix and Its Components.

Tissue engineering. Part A·2015
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Pyrosequencing offers a thorough and simple method for analyzing genetic polymorphisms after the Human Genome Project. This technique enables rapid single-nucleotide polymorphism evaluation and assessment of various genetic variations.

Area of Science:

  • Genetics
  • Molecular Biology
  • Bioinformatics

Background:

  • The Human Genome Project has generated vast genetic data, necessitating advanced genotyping methods.
  • Analyzing genetic polymorphisms is crucial for pharmacogenetic research.

Purpose of the Study:

  • To explain the technique and methodology of Pyrosequencing.
  • To highlight Pyrosequencing as a robust method for genetic polymorphism analysis.

Main Methods:

  • Pyrosequencing utilizes sequencing by synthesis to analyze DNA.
  • The method allows for the identification of single-nucleotide polymorphisms, tri-allelic sites, insertions/deletions (indels), and short-repeat polymorphisms.
  • Allele quantification for methylation analysis or pooled samples is also possible.

Related Experiment Videos

Main Results:

  • Pyrosequencing provides thorough and simple analysis of genetic polymorphisms.
  • It enables rapid and efficient evaluation of single-nucleotide polymorphisms, including clinically relevant ones.
  • Internal quality control is facilitated by a standardized control sequence.

Conclusions:

  • Pyrosequencing is a valuable tool for pharmacogenetics, leveraging data from the Human Genome Project.
  • The method's versatility supports diverse genetic analyses, including complex variations and pooled samples.
  • Its efficiency and accuracy make it suitable for evaluating clinically relevant polymorphisms.