Related Experiment Video

Updated: Jul 18, 2026

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
06:59

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue

Published on: October 12, 2013

CAM5.2-positive subserosal myofibroblasts in appendicitis

Tadashi Hamauzu1, Naoto Kuroda, Limei Guo

  • 1Department of Pathology, Program of Bioregulation and Genetics, Kochi Medical School, Kochi University, Kochi, Japan.

Medical Molecular Morphology
|December 26, 2006
PubMed

Insights

Myofibroblasts accumulate around appendicitis perforations, originating from submesothelial or mesothelial cells. These cells, identified by specific markers, replace absent CD34-positive stromal cells in the affected area.

Area of Science:

  • Gastroenterology
  • Surgical Pathology
  • Cell Biology

Background:

  • Appendicitis perforation leads to significant tissue changes.
  • The role of myofibroblasts in appendicitis complications is not fully understood.
  • Understanding stromal cell origin is crucial for tissue repair mechanisms.

Purpose of the Study:

  • To investigate the distribution and origin of myofibroblasts in appendicitis perforations.
  • To characterize stromal cell populations in normal and perforated appendices.
  • To identify potential cellular sources of myofibroblasts in appendicitis.

Main Methods:

  • Immunohistochemistry on 45 appendicitis cases.
  • Analysis of stromal cells using markers like alpha-smooth muscle actin (ASMA), CD34, cytokeratin CAM5.2, cytokeratin 5, and HBME-1.
  • Double immunostaining to assess coexpression of markers.

Main Results:

  • Myofibroblasts were found in the subserosal area around appendicitis perforations, particularly with abscess and granulation tissue.
  • CD34-positive stromal cells were absent in the perforation's subserosal area.
  • Coexpression of ASMA and cytokeratin CAM5.2, and cytokeratin 5 and cytokeratin CAM5.2, was observed in stromal cells.

Conclusions:

  • Myofibroblasts in the subserosal area of appendicitis perforations likely originate from submesothelial or mesothelial cells.
  • These findings highlight a distinct cellular response in appendicitis complications.
  • The study provides insights into the cellular dynamics of wound healing in appendicitis.

More Related Videos

Murine Appendectomy Model of Chronic Colitis Associated Colorectal Cancer by Precise Localization of Caecal Patch08:51

Murine Appendectomy Model of Chronic Colitis Associated Colorectal Cancer by Precise Localization of Caecal Patch

Published on: August 24, 2019

Isolation of CD 90+ Fibroblast/Myofibroblasts from Human Frozen Gastrointestinal Specimens06:02

Isolation of CD 90+ Fibroblast/Myofibroblasts from Human Frozen Gastrointestinal Specimens

Published on: January 31, 2016

Related Concept Videos

Appendicitis01:19

Appendicitis

Appendicitis is an acute inflammatory condition of the vermiform appendix, most commonly caused by obstruction of its lumen. The appendix is a narrow, blind-ended pouch that extends from the cecum, making it particularly prone to obstruction. Causes include fecaliths, lymphoid hyperplasia (often after viral infections), parasites, tumors, or foreign bodies. This obstruction initiates a cascade of pathological changes.Luminal Obstruction and Early InflammationAfter obstruction, normal mucosal...
Appendicitis-I: Introduction01:22

Appendicitis-I: Introduction

The appendix, a small, narrow, blind tube extending from the inferior part of the cecum, is widely regarded as a vestigial organ, having lost much of its original function through evolution. Despite its diminished role, the appendix can become inflamed, a condition known as appendicitis.
Etiology: Appendicitis can arise from various causes, primarily rooted in the obstruction of the appendix lumen. Factors contributing to this obstruction include fecal accumulation, lymphoid hyperplasia and, in...
Appendicitis-II: Diagnostic Studies and Management01:29

Appendicitis-II: Diagnostic Studies and Management

Diagnosing and managing appendicitis requires a structured and comprehensive approach that spans from initial assessment to postoperative care. Here is an overview of the process:
Diagnosing Appendicitis
It requires a multifaceted approach, starting with a detailed physical examination to pinpoint the location and nature of the pain and identify any associated symptoms. Laboratory tests play a crucial role. A complete Blood Count (CBC) typically reveals leukocytosis (an increased number of...
The Role of Actin and Myosin in Non-muscle Cells01:10

The Role of Actin and Myosin in Non-muscle Cells

Actin and myosin or actomyosin filaments also play a significant role in cells other than those involved in muscle contraction (which occurs within the sarcomere of muscle cells). The mechanism of non-muscle cell contractile bundles was first observed in Dictyostelium and Acanthamoeba. In non-muscle cells, two bundles are commonly found: stress fibers and actomyosin adherence belts. These contractile bundles are smaller and less organized than the ones found in muscle cells. They  are held...
Diverticular Disease of the Colon01:27

Diverticular Disease of the Colon

Diverticular disease involves the formation of diverticula—small sac-like outpouchings of the colonic wall—and their complications. It most commonly affects the sigmoid colon due to higher intraluminal pressure and structural vulnerability. It results from structural weakness and increased pressure in the colon, producing pseudodiverticula that may remain silent or progress to inflammation and serious complications.Structure of DiverticulaIn diverticulosis, these outpouchings are...