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Updated: Jul 18, 2026
The Generation of Closed Femoral Fractures in Mice: A Model to Study Bone Healing
Published on: August 16, 2018
Young and middle-age associated differences in cytokeratin expression after bone fracture, tissue trauma, and
Takeshi Matsutani1, Shih-Ching Kang, Masao Miyashita
1Center for Surgical Research and Department of Surgery, University of Alabama at Birmingham, G 094 Volker Hall, 1670 University Blvd, Birmingham, AL 35294-0019, USA.
Background:
This study examined whether there is any difference in the lung cytokine and cytokeratin 19 levels between young and middle-aged mice after bone fracture and soft-tissue trauma hemorrhage (Fx-TH).
Methods:
Young (6-8 weeks) and middle-aged (12 months) C3H/HeN male mice were subjected to right lower leg fracture, trauma hemorrhage (mean arterial blood pressure to 35 +/- 5 mm Hg for 90 minutes), and resuscitation.
Results:
The tumor necrosis factor alpha level in the lung increased significantly at 2 hours after Fx-TH in both young and middle-aged mice, whereas at 24 hours the levels remained significantly higher in middle-aged mice. Interleukin-6 levels increased significantly 24 hours after Fx-TH in both groups, whereas interleukin-10 levels increased only in middle-aged mice at 24 hours under those conditions. Monocyte chemoattractant protein-1 levels increased significantly 2 hours after Fx-TH. The protein and messenger RNA levels of cytokeratin 19 were significantly higher in middle-aged mice compared with young mice after Fx-TH.
Conclusion:
These results suggest that age influences the lung inflammatory response after Fx-TH.
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