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The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
13:02

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Published on: October 5, 2016

The diversity of pathogenesis-related proteins decreases during grape maturation.

Sara Monteiro1, Maria A Piçarra-Pereira, Virgílio B Loureiro

  • 1Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, 2781-901 Oeiras, Portugal. smonteiro@isa.utl.pt

Phytochemistry
|December 26, 2006
PubMed
Summary
This summary is machine-generated.

Grape pathogenesis-related (PR) proteins are synthesized in diverse forms early in development, not from a single precursor. Their diversity decreases as grapes mature, contrary to previous wine protein hypotheses.

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Area of Science:

  • Plant Biochemistry
  • Enology
  • Molecular Biology

Background:

  • Wines contain diverse, structurally similar polypeptides homologous to pathogenesis-related (PR) proteins.
  • A prior hypothesis suggested these wine PR proteins originate from one or few common precursors in mature grapes.

Purpose of the Study:

  • To investigate the origin and diversity of pathogenesis-related (PR) proteins during grape berry development.
  • To test the hypothesis that wine PR proteins arise from limited precursors.

Main Methods:

  • Analysis of PR protein patterns in grape berries from early development to maturity.
  • Utilized 2-D immunoblotting and FPLC (Fast Protein Liquid Chromatography) coupled with 1-D immunoblotting.

Main Results:

  • PR protein concentration increases during grape berry development.
  • PR protein diversity decreases from early development to maturity.
  • Contrary to the precursor hypothesis, PR proteins are synthesized in various forms early on.

Conclusions:

  • The hypothesis of one or few common precursors for wine PR proteins is eliminated.
  • Pathogenesis-related proteins exhibit diverse synthesis patterns from early grape development stages.