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Serological analysis of an oncornavirus (PMF virus) detected in malignant permanent human cell lines
Abstract:
Immunodiffusion analysis of the PMF virus which was detected in malignant permanent human cell lines revealed positive reactions with antisera against the Mason-Pfizer monkey virus (MPMV). No cross-reactivity was demonstrated with murine leukemia virus (MuLV), rat leukemia virus (RaLV), hamster leukemia virus (HaLV), feline leukemia virus (FeLV), simian (woolly monkey) sarcoma virus (SSV-1) and mouse mammary tumor virus (MTV). The cross-reactive antigens of the PMF virus and the MPMV are considered as evidence for the human origin of the PMF virus.
Insights
The PMF virus found in human cell lines reacted with Mason-Pfizer monkey virus (MPMV) antisera. This cross-reactivity suggests a human origin for the PMF virus, differentiating it from other retroviruses.
Area of Science:
- Virology
- Oncology
- Molecular Biology
Background:
- The presence of novel viruses in human cell lines necessitates thorough characterization.
- Distinguishing between endogenous and exogenous viral origins is crucial in cancer research.
Purpose of the Study:
- To identify the viral etiology of the PMF virus detected in human malignant cell lines.
- To determine the antigenic relationship of the PMF virus to known retroviruses.
Main Methods:
- Immunodiffusion assays were performed.
- Antisera against Mason-Pfizer monkey virus (MPMV) and various other retroviruses were used.
- Reactions with PMF virus isolates from human cell lines were analyzed.
Main Results:
- PMF virus isolates showed positive immunodiffusion reactions exclusively with MPMV antisera.
- No cross-reactivity was observed with antisera against murine leukemia virus (MuLV), rat leukemia virus (RaLV), hamster leukemia virus (HaLV), feline leukemia virus (FeLV), simian (woolly monkey) sarcoma virus (SSV-1), or mouse mammary tumor virus (MTV).
Conclusions:
- The observed cross-reactivity between PMF virus and MPMV provides strong evidence for the human origin of the PMF virus.
- This finding aids in understanding the potential role of specific retroviruses in human malignancies.