Measurement of antilymphokine serum activities by the electrophoretic mobility test

Purified human lymphocytes from peripheral blood were stimulated by Con A and the concentrated supernatants were used to induce antibodies in rabbits. These antilymphokine immune sera (ALKS) were able to inhibit the electrophoretic mobility of indicator cells, which was performed using supernatants of lymphocytes stimulated by Con A or PPD and tanned sheep red blood cells as indicator particles. If the method is standardized it is possible to compare several ALKS from different origin even in different models.