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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
Conservation of Protein Domains02:26

Conservation of Protein Domains

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...

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Related Experiment Video

Updated: Jul 18, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Overcoming protein diversity on arrays.

Nicole Rusk

    Nature Methods
    |December 27, 2006
    PubMed
    Summary

    To accurately map protein interactions using microarray data, researchers found that measuring saturation binding curves is essential. This method ensures reliable protein interaction network establishment for each protein and its partners.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Bioinformatics

    Background:

    • Protein interaction networks are crucial for understanding cellular functions.
    • Microarray technology offers a high-throughput approach for studying these interactions.

    Purpose of the Study:

    • To determine the necessary conditions for reliably establishing protein interaction networks using microarray data.
    • To identify key experimental parameters for accurate protein binding analysis.

    Main Methods:

    • The study involved analyzing microarray data to assess protein interactions.
    • Saturation binding curves were measured for arrayed proteins and their potential binding partners.

    Main Results:

    • Reliable establishment of protein interaction networks requires measuring saturation binding curves.

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  • This measurement is necessary for each arrayed protein and its potential binding partners.

    • Conclusions:

    • Saturation binding curve measurements are a critical step for accurate protein interaction network construction from microarray data.
    • Implementing this method enhances the reliability of high-throughput protein interaction studies.