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Updated: May 3, 2026

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Published on: October 11, 2013

High-throughput fluorescent multiplex array for indoor allergen exposure assessment.

Christopher D Earle¹, Eva M King, Amy Tsay

¹Indoor Biotechnologies, Inc, Charlottesville, VA 22903, USA. cearle@inbio.com <cearle@inbio.com>

The Journal of Allergy and Clinical Immunology

|January 2, 2007

Summary

This summary is machine-generated.

A new fluorescent multiplex array allows simultaneous detection of six indoor allergens, improving efficiency over traditional methods. This advancement aids in large-scale allergen exposure studies and environmental assessments.

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Area of Science:

Biotechnology
Immunology
Environmental Science

Background:

Current enzyme immunoassay methods for indoor allergen detection are inefficient.
Existing methods are labor-intensive and time-consuming for analyzing environmental dust samples.

Purpose of the Study:

To develop and validate a novel fluorescent multiplex array.
To enable simultaneous measurement of six key indoor allergens: Der p 1, Der f 1, Der p 2, Der f 2, Fel d 1, and Can f 1.

Main Methods:

Developed a multiplex array using monoclonal antibodies (mAbs) covalently linked to fluorescent microspheres.
Utilized a single universal standard of purified natural allergens for array development.
Validated the array by comparing results with standard ELISA methods for dust mite, cat, and dog allergens in household dust.

Main Results:

Demonstrated highly significant quantitative correlation (R² 0.90–0.99, P < .001) between the multiplex array and ELISA.
Achieved superior or equivalent sensitivity, limit of detection (<0.1 ng/mL), and reproducibility (intra-assay CV <5%, interassay CV <25%) compared to ELISA.

Conclusions:

Successfully developed a multiplex array for simultaneous measurement of six indoor allergens from single samples.
The array is suitable for epidemiological studies, indoor air quality assessments, and clinical/population-based environmental surveys.
The platform is adaptable for inclusion of additional allergens and biologics.