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Related Experiment Video

Updated: Jul 18, 2026

Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide

Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide

Published on: May 12, 2023

Steroidogenic factor-1 is a sphingolipid binding protein.

Aarti N Urs¹, Eric Dammer, Samuel Kelly

¹School of Biology and the Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, 310 Ferst Drive, Atlanta, GA 30332-0230, United States.

Molecular and Cellular Endocrinology

|January 2, 2007

Summary

Sphingosine is identified as an endogenous ligand for Steroidogenic Factor 1 (SF1), a key regulator of steroid hormone biosynthesis. This discovery suggests bioactive lipids play a crucial role in controlling SF1 activity and hormone production.

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Last Updated: Jul 18, 2026

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A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

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Published on: June 8, 2022

Area of Science:

Endocrinology
Molecular Biology
Biochemistry

Background:

Steroidogenic factor (SF1) is crucial for steroid hormone biosynthesis and endocrine development.
SF1 activity is regulated by post-translational modifications, localization, and protein interactions.
Ligand binding is critical for controlling SF1 transactivation activity.

Purpose of the Study:

To identify endogenous ligands for SF1.
To elucidate the role of bioactive lipids in SF1-mediated steroidogenesis.

Main Methods:

Mass spectrometric analysis of endogenous SF1.
Crystallographic studies of SF1.
Investigation of SF1-ligand interactions.

Main Results:

Sphingosine was identified as an endogenous ligand for SF1.
SF1 can bind both sphingolipids and phospholipids.
Multiple bioactive lipids likely act as SF1 ligands, modulating its activity differentially.

Conclusions:

Bioactive lipids, including sphingosine, are endogenous ligands for SF1.
These lipids differentially regulate SF1 activity in a context-dependent manner.
Bioactive lipids are central mediators of trophic hormone-stimulated steroid hormone biosynthesis.