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Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose
Greg B G Moorhead1, Timothy A J Haystead, Carol MacKintosh
1Department of Biological Sciences, University of Calgary, Alberta, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|January 4, 2007
Abstract:
Microcystin-based affinity matrices have been utilized to demonstrate the association of signaling proteins with protein phosphatases and for the purification of low-abundance microcystin-sensitive protein phosphatases. Here, we describe the procedure for the synthesis and use of microcystin-Sepharose and microcystin-biotin-Sepharose.
