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Assay for three-way interaction of protein phosphatase-1 (Glc7) with regulatory subunits plus phosphatase inhibitor-2
Masumi Eto1, David L Brautigan
1Department of Physiology, Thomas Jefferson College of Medicine, Philadelphia, PA, USA.
Abstract:
A method is described using yeast conjugation to assay the interactions of a protein phosphatase-1 (PP1) inhibitor protein with holoenzymes formed in situ by expression of regulatory subunit fusion proteins that recruit endogenous Glc7, the yeast ortholog of PP1. Mutations in the canonical recognition motif VxF used to bind PP1 (Glc7) allow for analysis of direct from indirect (three-way) interactions.
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