Related Experiment Video
Updated: Jul 17, 2026
09:32
ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
Published on: June 9, 2011
Time-resolved fluoroimmunoassay for ractopamine in swine tissue
Jianzhong Shen1, Zhen Zhang, Yan Yao
1Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China.
Analytical and Bioanalytical Chemistry
|January 4, 2007
Summary
A new time-resolved fluoroimmunoassay (TR-FIA) effectively screens for ractopamine (RAC) in swine tissue. This validated method offers accurate detection for ensuring meat safety.
Area of Science:
- Analytical Chemistry
- Food Safety Science
- Biotechnology
Background:
- Ractopamine (RAC) is a widely used feed additive in livestock production.
- Accurate detection methods are crucial for monitoring RAC residues in food products.
- Existing methods may require complex sample preparation or expensive equipment.
Purpose of the Study:
- To develop and validate a rapid and sensitive time-resolved fluoroimmunoassay (TR-FIA) for screening RAC in swine tissue.
- To establish a reliable method for detecting RAC residues at low concentrations.
- To confirm the method's applicability in real-world residue studies.
Main Methods:
- Development of a direct competitive-type immunoassay.
- Utilized europium-labeled anti-RAC monoclonal antibody as a tracer.
- Employed RAC-ovalbumin as a solid-phase antigen for assay immobilization.
Main Results:
- Achieved high recovery rates (88.2-118.5%) for RAC in swine liver and muscle.
- Demonstrated low coefficients of variation (7.1-20.5%), indicating method precision.
- Established a sensitive detection limit of 0.1 microg kg(-1) for RAC.
Conclusions:
- The developed TR-FIA method is suitable for screening RAC in swine tissue.
- The method's applicability was confirmed through an actual residue study.
- TR-FIA offers a viable alternative for routine monitoring of RAC residues.
