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[Expression of Bax and Bcl-2 gene in rat testis tissues induced by testicular denervation]
Yong-guang Gong1, Yu-ru Yang, Wei Zhagn
1Department of Urology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029 China. gongyongguang@sohu.com
Objective:
To detect the changes of the expression of Bax and Bcl-2 gene in the denervated testis, and to explore the possible mechanisms underlying the apoptosis of germ cells induced by testicular denervation at the genetic translation level.
Methods:
Eighteen mature SD rats (350-375 g) were equally divided into 3 groups: a sham operation group( SO) , a superior spermatic nerve group (SSN) and an inferior spermatic nerve group (ISN) , and the latter two received bilateral surgical removal of the superior spermatic nerve and the inferior spermatic nerve, respectively. The animals were killed I month after the operation. ISH SP-method was used to detect the expression of Bax and Bcl-2 protein.
Results:
Significant up-regulation of Bax protein was detected in both the treatment groups 1 month after surgery( P <0. 05) , and the level of Bcl-2 protein remained unchanged.
Conclusion:
Bax gene is involved in the apoptosis of germ cells induced by testicular denervation.
