Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Fibril-associated Collagen

Fibril-associated Collagen

Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...

Cell-matrix's Response to Mechanical Forces

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Field-Based Planetary Protection Operations for Melt Probes: Validation of Clean Access into the Blood Falls, Antarctica, Englacial Ecosystem.

Astrobiology·2023

Same author

Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix.

Materials science & engineering. C, Materials for biological applications·2019

Same author

Crystal structure, phase transitions, and magnetic properties of iridium perovskites Sr2MIrO6 (M = Ni, Zn).

Inorganic chemistry·2013

Same author

Ferromagnetic Cu-O-Cu coupling in CaCu3Sn4O12 probed by neutron diffraction.

Journal of physics. Condensed matter : an Institute of Physics journal·2012

Same author

Thermal fluctuations of haemoglobin from different species: adaptation to temperature via conformational dynamics.

Journal of the Royal Society, Interface·2012

Same author

Molecular processes in biological thermosensation.

Journal of biophysics (Hindawi Publishing Corporation : Online)·2010

Search research articles

Related Experiment Video

Updated: Jul 17, 2026

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Published on: August 22, 2016

Decrease in extracellular collagen crosslinking after NMR magnetic field application in skin fibroblasts.

I Digel¹, E Kurulgan, Pt Linder

¹Department of Biomedical Engineering, Laboratory of Cell Biophysics, Aachen University of Applied Sciences, Ginsterweg 1, 52428, Juelich, Germany. digel@fh-aachen.de

Medical & Biological Engineering & Computing

|January 5, 2007

Summary

Nuclear magnetic resonance (NMR) magnetic field exposure altered human dermal fibroblast extracellular matrix, reducing collagen cross-linking. These findings suggest potential therapeutic applications for electromagnetic fields in skin tissue engineering.

More Related Videos

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

Published on: May 1, 2020

Related Experiment Videos

Last Updated: Jul 17, 2026

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Published on: August 22, 2016

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

Published on: May 1, 2020

Area of Science:

Biophysics
Cell Biology
Biomaterials Science

Background:

The biological impact of electromagnetic fields (EMFs) remains incompletely understood, with ongoing debate regarding their significance and mechanisms.
Therapeutic applications of EMFs are largely unexplored due to a lack of consensus on their effects.

Purpose of the Study:

To investigate the effects of magnetic fields under nuclear magnetic resonance (NMR) conditions on human dermal fibroblasts.
To analyze changes in cellular and extracellular matrix components, including collagen types I, III, and IV.

Main Methods:

Primary human dermal fibroblast cultures were exposed to a magnetic field at NMR conditions for 5 days (4 h/day).
Assessed parameters included cell proliferation, morphology, total protein concentration, and collagen content (types I, III, IV).
Compared effects with magnetic field exposure in the absence of resonance frequency.

Main Results:

NMR exposure induced significant alterations in both cellular and extracellular components of fibroblasts.
A notable decrease in collagen cross-linking within the extracellular matrix was observed.
Increased soluble collagen fractions were quantified: 17.2% for type I, 27.0% for type III, and 17.3% for type IV.

Conclusions:

Magnetic fields at NMR conditions demonstrably affect fibroblast extracellular matrix composition, specifically reducing collagen cross-linking.
The observed changes suggest EMFs, particularly under resonance conditions, could influence tissue structure.
Effects were less pronounced when magnetic field exposure lacked resonance frequency, highlighting the importance of specific frequencies.