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Improved embryonic stem cell technologies.

J S Draper¹, A Nagy

¹Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada. draper@mshri.on.ca

Handbook of Experimental Pharmacology

|January 6, 2007

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Mouse embryonic stem (ES) cells are vital for studying gene function. New technologies allow precise genetic manipulation for advanced research in vivo and in vitro.

Area of Science:

Developmental Biology
Genetics
Stem Cell Biology

Background:

Murine embryonic stem (ES) cells are crucial for genetic research.
Technological advancements have enhanced ES cell capabilities.

Purpose of the Study:

To review the history and recent progress in mouse ES cell technology.
To highlight advancements in ES cell derivation and genetic manipulation.

Main Methods:

Tetraploid aggregation for ES cell manipulation.
Site-specific recombinases for targeted genetic modification.
RNA interference (RNAi) for gene function studies.

Main Results:

Sophisticated manipulation of the ES cell genome is now possible.

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Temporal and spatial control of gene expression is achievable.

Complex gene function and interaction questions can be addressed.

Conclusions:

Mouse ES cells are indispensable tools for genetic research.
Recent technological progress has significantly advanced ES cell applications.
ES cell-derived chimaeric animals are key for in vivo studies.